5
Anti-His-tag Mouse Monoclonal Antibody
分类 工具抗体
参数
货号：SLS-KY1001X
克隆号：MC10
来源：Murine ascites
免疫原：Synthetic HHHHHH peptide conjugated to KLH as immunogen.
亚型：IgG1
规格：100ul/vial or 1ml/vial
状态和缓冲液：0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)
纯度：>95% (SDS-PAGE)
保存条件：Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.
用途：Western Blot (WB)、Immunofluorescence (IF)、Immunoprecipitation (IP)
使用建议：Western Blot (WB): 1:1000—1:10000 Immunofluorescence (IF): 1:100 — 1:1000 Immunoprecipitation (IP): 1:100 — 1:1000
实验照片
Product Description:
The His-tag is a fusion tag consisting of six histidine residues, widely used for the detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight that generally does not affect target protein function; strong binding affinity between histidine and immobilized nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that specifically recognizes His sequences at the N-terminus and C-terminus of fusion proteins.
Notes:
1.Use an anti-mouse secondary antibody, e.g., Goat anti-Mouse IgG (H+L)-HRP (SLS-KY3001).
2.Determine the optimal antibody dilution for your specific experiment.
3.For research use only; not for clinical diagnosis.
描述
Product Description:
The His-tag is a fusion tag consisting of six histidine residues, widely used for the detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight that generally does not affect target protein function; strong binding affinity between histidine and immobilized nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that specifically recognizes His sequences at the N-terminus and C-terminus of fusion proteins.
Notes:
1.Use an anti-mouse secondary antibody, e.g., Goat anti-Mouse IgG (H+L)-HRP (SLS-KY3001).
2.Determine the optimal antibody dilution for your specific experiment.
3.For research use only; not for clinical diagnosis.
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