5
Anti-His-tag HRP Conjugated Mouse Monoclonal Antibody
分类 工具抗体
参数
货号：SLS-KY1101
来源：Murine ascites
免疫原：Synthetic HHHHHH peptide conjugated to KLH as immunogen.
克隆号：MC10
亚型：IgG1
规格：100ul/vial or 1ml/vial
状态和缓冲液：0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)
纯度：>95% (SDS-PAGE)
保存条件：Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.
保存时间：3 Years
用途：Western Blot (WB)
使用建议：Western Blot (WB): 1:1000—1:10000
实验照片
Product Description:
His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.
Notes:
1.This product is HRP-conjugated and can be directly visualized after incubation and washing.
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
描述
Product Description:
His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.
Notes:
1.This product is HRP-conjugated and can be directly visualized after incubation and washing.
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
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