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Anti-His-tag HRP Conjugated Mouse Monoclonal Antibody

分类 工具抗体

参数

货号:SLS-KY1101

克隆号:MC10

来源:Murine ascites

免疫原:Synthetic HHHHHH peptide conjugated to KLH as immunogen.

亚型:IgG1

规格:100ul/vial or 1ml/vial

状态和缓冲液:0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)

纯度:>95% (SDS-PAGE)

保存条件:Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.

用途:Western Blot (WB)

使用建议:Western Blot (WB): 1:1000—1:10000

实验照片

Anti-His-tag HRP Conjugated Mouse Monoclonal Antibody


Product Description: 



His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.





Notes:



1.This product is HRP-conjugated and can be directly visualized after incubation and washing.



2.Please determine the optimal antibody dilution according to your experiment.



3.This product is for scientific research use only, not for clinical diagnosis.





描述


Product Description: 



His refers to a fusion tag composed of six histidines, which is widely used for the expression detection and purification of fusion proteins. Advantages of the His-tag include: small molecular weight, which generally does not affect the function of the target protein; strong adsorption of histidine to solid-phase nickel, allowing His-tagged proteins to be purified using Ni columns even under denaturing conditions. Our His antibody is a highly purified monoclonal antibody that can specifically recognize His sequences at both the N-terminus and C-terminus of fusion proteins.





Notes:



1.This product is HRP-conjugated and can be directly visualized after incubation and washing.



2.Please determine the optimal antibody dilution according to your experiment.



3.This product is for scientific research use only, not for clinical diagnosis.





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