5
Anti Flag-tag Mouse Monoclonal Antibody
分类 工具抗体
参数
货号：SLS-KY1002X
来源：Murine ascites
免疫原：Synthetic DYKDDDDK conjugated to KLH was used as the immunogen.
克隆号：MA4
亚型：IgG1
规格：100ul/vial or 1ml/vial
状态和缓冲液：0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)
纯度：>95% (SDS-PAGE)
保存条件：Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.
保存时间：3 Years
用途：Western Blot (WB)、Immunofluorescence (IF)、Immunoprecipitation (IP)
使用建议：Western Blot (WB): 1:1000—1:10000 Immunofluorescence (IF): 1:100 — 1:1000 Immunoprecipitation (IP): 1:100 — 1:1000
实验照片
Product Description:
The Flag-tag protein consists of a hydrophilic polypeptide encoded by 8 amino acids and is widely used for the detection and purification of fusion protein expression. The advantages of the Flag-tag include: small molecular weight, which generally does not affect the function of the target protein; the active target protein can be obtained by affinity purification with Flag antibody. The Flag antibody of our company is a highly purified monoclonal antibody that can specifically recognize the Flag sequence at the N-terminal, C-terminal and intermediate positions of the fusion protein with high specificity.
Notes:
1.Please use an anti-mouse secondary antibody, e.g., Goat anti-Mouse IgG(H+L)-HRP (SLS-KY3001).
2.Determine the optimal antibody dilution according to experimental conditions.
3.This product is for scientific research use only and not for clinical diagnosis.
描述
Product Description:
The Flag-tag protein consists of a hydrophilic polypeptide encoded by 8 amino acids and is widely used for the detection and purification of fusion protein expression. The advantages of the Flag-tag include: small molecular weight, which generally does not affect the function of the target protein; the active target protein can be obtained by affinity purification with Flag antibody. The Flag antibody of our company is a highly purified monoclonal antibody that can specifically recognize the Flag sequence at the N-terminal, C-terminal and intermediate positions of the fusion protein with high specificity.
Notes:
1.Please use an anti-mouse secondary antibody, e.g., Goat anti-Mouse IgG(H+L)-HRP (SLS-KY3001).
2.Determine the optimal antibody dilution according to experimental conditions.
3.This product is for scientific research use only and not for clinical diagnosis.
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