5
Anti-Myc-tag Mouse Monoclonal Antibody
分类 工具抗体
参数
货号：SLS-KY1003
克隆号：ME8
来源：Murine ascites
免疫原：Synthetic EQKLISEEDL peptide conjugated to KLH as immunogen.
亚型：IgG1
规格：100ul/vial or 1ml/vial
状态和缓冲液：0.01M pH7.4 PBS, 0.1%BSA, 50% Glycerol(0.02% Sodium azide)
纯度：>95% (SDS-PAGE)
保存条件：Stored at 2-8℃ short term, -20℃ or colder long term. Avoid multiple freeze/thaw cycles.
用途：Western Blot (WB)、Immunofluorescence (IF)、Immunoprecipitation (IP)
使用建议：Western Blot (WB): 1:1000—1:10000 Immunofluorescence (IF): 1:100 – 1:1000 Immunoprecipitation (IP): 1:100 – 1:1000
实验照片
Product Description:
The Myc-tag is derived from amino acids 410–419 of human c-Myc protein. The Myc-tag can be placed at the N-terminus or C-terminus. However, the low-pH elution conditions for Myc-tagged recombinant proteins often reduce protein activity, so the Myc-tag system is widely used for detection but rarely for purification. The Myc-tag has been successfully applied in Western blot (WB), immunoprecipitation (IP), and flow cytometry. Our Myc antibody is a highly purified monoclonal antibody that specifically recognizes Myc sequences at the N-terminus, C-terminus, and internal positions of fusion proteins.
Notes:
1.Please use an anti-mouse secondary antibody, such as Goat anti-Mouse IgG (H+L)-HRP (SLS-KY3001).
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
描述
Product Description:
The Myc-tag is derived from amino acids 410–419 of human c-Myc protein. The Myc-tag can be placed at the N-terminus or C-terminus. However, the low-pH elution conditions for Myc-tagged recombinant proteins often reduce protein activity, so the Myc-tag system is widely used for detection but rarely for purification. The Myc-tag has been successfully applied in Western blot (WB), immunoprecipitation (IP), and flow cytometry. Our Myc antibody is a highly purified monoclonal antibody that specifically recognizes Myc sequences at the N-terminus, C-terminus, and internal positions of fusion proteins.
Notes:
1.Please use an anti-mouse secondary antibody, such as Goat anti-Mouse IgG (H+L)-HRP (SLS-KY3001).
2.Please determine the optimal antibody dilution according to your experiment.
3.This product is for scientific research use only, not for clinical diagnosis.
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